RiboLace

IMMAGINA’s proprietary RiboLace^TM technology enables fast and selective extraction of ribosomes in active translation (both in vitro and in vivo) and purification of ribosome protected fragments (RPFs) for the isolation of active Ribosome Protected Fragments. At the heart of the method lies a proprietary puromycin derivative, called 3P, that retains the ability to interact with active ribosomes while being covalently linked to a biotin molecule. The samples are first exposed to cycloheximide to clamp ribosomes on the mRNA fragments (recommended step), then they are lysed and nuclease digested to produce the RPF. In parallel, magnetic beads are functionalized with the 3P, and once the digestion is over, they are added to the reaction mix to pull down the active ribosomal complex. Eventually, the RPFs are isolated from the ribosomal complex by simple differential elution.

ALL-IN-ONE RiboLace Gel Free (12 rxns)

This all-inclusive RiboSeq kit streamlines your workflow by skipping gel extraction. This not only saves you time but also potentially increases the amount of valuable information you can obtain from your samples.

ALL-IN-ONE RiboLace Multiplexing

This multiplexed RiboSeq kit streamlines your workflow by handling 1 instead of 6 samples and permits minimal input, allowing low translating samples. This saves you time and potentially increases the amount of valuable information you can obtain from your samples.

ALL-IN-ONE RiboLacePro (12 rxns)

This ready-to-use kit offers a complete RiboSeq solution, including all reagents necessary for RPF gel extraction and library preparation from your sample.

RiboLace 360 Gel Free Kit (12 rxns)

A sample-to-data RiboSeq workflow that combines wet-lab reagents, Sequencing service, and Data Analysis service in one single and easy-to-use research solution.

RiboLace 360 TE Gel Free Kit (12 rxns)

A sample-to-data RiboSeq and RNASeq workflow to assess translation efficiency of the specimen of interest that combines wet-lab reagents, Sequencing service, and Data Analysis service in one single and easy-to-use research solution.

RiboLace Pro (12 rxns)

The RiboLace Pro kit contains all reagents to isolate active ribosomes by affinity purification and magnetic separation.

RiboLace Starter (4 rxns)

RiboLace Starter Kit: isolate active ribosomes for new users or samples of unknown tranlsational activity. Adjustable protocol lets you customize experiments

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RiboLace

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RiboLace