tRNA sequencing

Total and ribo-embedded full-length tRNA capture

nano-tRNAseq

Quantification and detection of tRNA modifications

Studying the function of tRNAs in protein translation is fundamental for understanding their role in several cellular processes that can be impaired in various diseases. Immagina's nano-tRNAseq is the only technology that simultaneously quantifies full-length, native-state tRNAs and detects chemical modifications in a single experiment.

Ribo-tRNAseq

Detection and quantification of ribo-embedded tRNA

Once inaccessible, ribo-embedded tRNAs are now at reach with Immagina’s service. Ribo-tRNAseq leverages our RiboLace technology to pulldown ribosomes in active translation and our cutting-edge tRNA sequencing technology. Ribo-tRNAseq can be used for studying tRNAs actively engaged in translation, especially relevant in the fields of oncology and viral infections.

Key characteristics

nano-tRNAseq ribo-tRNAseq
Captured tRNAs Total tRNA population (full-length, fragments, etc) tRNAs actively engaged in translation
Input
  • All species: > 3 µg of total RNA
  • Eukaryotic cell lines: > 0.5 M
  • Tissues: > 10mg
tRNA abundance detection?
tRNA modifications detection?