Ribo-tRNAseq

Studying the function of actively used tRNAs in protein translation is fundamental for understanding their role in several cellular processes that can be impaired in various diseases. Immagina’s ribo-tRNAseq leverages our active ribosome pulldown technology, RiboLace, with our tRNA sequencing technology to isolate and sequence ribo-embedded tRNAs. In a single run, our method simultaneously quantifies full-length, native-state tRNAs and detects chemical modifications.

Service overview

Our service ties the pioneering work at the laboratory of Dr. Eva Novoa (Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology, Barcelona, Spain with our own active ribosome pulldown innovation. In fact, a recent paper by Monti et al.¹ highlights the differences in total tRNA and ribo-tRNA pools upon different stress events, such as translation arrest and viral infection. Ribo-tRNAseq comprises the following steps:

RiboLace active ribosome pulldown
Small RNA isolation: enriched from total RNA
Library preparation: allowing multiplexing up to 6 samples
Sequencing on Oxford Nanopore platform
Data analysis

^{1 - Monti, M., Yilmaz, H., Del Piano, A., Arnoldi, M., Bonomo, I., Llovera, L., Sarabando, J., Ribeiro, D., Soares, A. R., Clamer, M., & Novoa, E. M. (2026). Selective Profiling of Translationally Active Trnas and Their Dynamics under Stress. https://doi.org/10.64898/2026.03.02.709006}

Technical applications

Uncover abundance and modifications of active tRNAs
Explore the role of actively used tRNAs in diseases
Reveal dynamic tRNA usage during translational shifts
tRNA charging status: upon request

Delivery time: 4 to 6 weeks

Service description and outcomes

Pre-service consultation to establish your experimental design
Input material: eukaryotic cell lines (>0.5M) and tissues (>10mg)
Complete and interactive bioinformatics report
Access to secure data transfer portal
Multiplexing up to 6 samples
High-efficiency data processing