CircAID-p-seq for Oxford Nanopore Technologies

 

CircAID-p-seq kit uses our proprietary CircAID-p-seq (Circular Amplification and IDentification of short RNA sequences bearing a 3′ Phosphate) technology, to generate Oxford Nanopore-compatible sequencing libraries without the need for PCR amplification. The kit is designed for quick (1-day) high-quality library preparation for short RNAs (20-50 nt) bearing a 3’-phosphate/2’,3’-cyclic phosphate (3’-P/2’,3’-cP) end. CircAID-p-seq (catalog no #CA001) includes all the components necessary for (i) first adaptor ligation, (ii) circularization, (iii) Reverse Transcription – Rolling Circle Amplification (RT-RCA). The final library product is suitable for next-generation sequencing (NGS) on the Oxford Nanopore platform, thus enabling real-time single-molecule detection of biologically relevant RNA species.

The sequencing output is analysed with an in-house computational pipeline called circAidMe available on-demand

Download the DATASHEET here

Applications:

o      RNA footprinting

o      Ribosome profiling

o      Transcriptome analysis

 

Shipping: Dry Ice
Storage condition: store component as indicated on data sheet
Shelf Life: 12 months
Protocol: #CA001 protocol

CircAID-p-seq is a patented technology