RiboNext 3PC (photo-active/alkyne puromycin)

3PBis is a UV-active alkyne analog of puromycin that efficiently binds to a network of proteins involved in ribosome function and activity (e.g. eEF1A, ENO1, GRP78).

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For research use only!
Shipping: shipped on dry ice
Quantity: 10 µL DMSO – 100 mM
Storage Conditions: Store at -80 °C.
Shelf Life: 3 months after the date of deliveryMolecular
Purity: ≥ 95 % (HPLC)
Form: DMSO solution
Solubility: DMSO, PBS (up to 100 µM tested)
Spectroscopic Properties: λmax 275 nm

Applications:
Monitoring of Protein synthesis activity – pull down of ribosome-associated proteins

Description:
A useful tool for sensing translation efficiency and selective deep-sequencing RNA analysis.

Selected References:
[1] Kandala D.T. et al. (2018) Sensing translation activity at the ribosome interface with UV-active small molecules. BioRxiv